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individual enzyme linked immunosorbent assay elisa kits kits r d systems inc dy1774  (R&D Systems)


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    R&D Systems individual enzyme linked immunosorbent assay elisa kits kits r d systems inc dy1774
    Individual Enzyme Linked Immunosorbent Assay Elisa Kits Kits R D Systems Inc Dy1774, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcp+1+elisa+kits/Canine+CCL2%2FMCP-1+DuoSet+ELISA/pmc13108725-70-25-31
    Average 93 stars, based on 4 article reviews
    individual enzyme linked immunosorbent assay elisa kits kits r d systems inc dy1774 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Cell Culture:

    Article Title: The TLR4-MyD88-NF-κB pathway is involved in sIgA-mediated IgA nephropathy
    Article Snippet: .. For detection of TNF-a, IL-6 and MCP-1 in cell culture supernatants, standard sandwich ELISA assays were performed using human TNF-a, IL-6 and MCP-1 ELISA kits (R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Role of Human Mesangial-Tubular Crosstalk in Secretory IgA-Induced IgA Nephropathy.
    Article Snippet: .. Enzyme-Linked Immunosorbent Assay To detect TNF-α, transforming growth factor-β1 (TGF-β1) and monocyte chemotactic protein-1 (MCP-1) levels in cell culture supernatants, standard sandwich ELISAs were performed using human TNF-α, TGF-β1, and MCP-1 ELISA kits (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s specifications. .. Real-Time Quantitative PCR Total cellular RNA was prepared using TRIZOL® Reagent (Invitrogen, Carlsbad, CA, USA) and used for cDNA synthesis with a reverse transcription kit (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions.

    Sandwich ELISA:

    Article Title: The TLR4-MyD88-NF-κB pathway is involved in sIgA-mediated IgA nephropathy
    Article Snippet: .. For detection of TNF-a, IL-6 and MCP-1 in cell culture supernatants, standard sandwich ELISA assays were performed using human TNF-a, IL-6 and MCP-1 ELISA kits (R&D Systems, Minneapolis, MN, USA). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: The TLR4-MyD88-NF-κB pathway is involved in sIgA-mediated IgA nephropathy
    Article Snippet: .. For detection of TNF-a, IL-6 and MCP-1 in cell culture supernatants, standard sandwich ELISA assays were performed using human TNF-a, IL-6 and MCP-1 ELISA kits (R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Down-regulation of miR-377 suppresses high glucose and hypoxia-induced angiogenesis and inflammation in human retinal endothelial cells by direct up-regulation of target gene SIRT1.
    Article Snippet: Diabetic retinopathy (DR) is one of the common microvascular complications of diabetes mellitus, which is the main cause of blindness in diabetic patients.. Angiogenesis plays an important role in retinal detachment and retinal microvascular inflammation throughout the whole development of DR.. This study aimed to investigate the regulatory effect and the potential mechanism of miR-377 on high glucose and hypoxia-induced angiogenesis and inflammation in human retinal endothelial cells, and found that the miR-377 level was significantly increased after high glucose and hypoxia-mimetic agent to simulate the DR milieu.

    Article Title: Ligustrazine ameliorates acute kidney injury through downregulation of NOD2‑mediated inflammation.
    Article Snippet: The second antibodies included HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) (cat. no. SA00001-1, 1:3,000, ProteinTech Group, Inc.) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) (cat. no. SA00001-2, 1:3,000, ProteinTech Group, Inc.). .. Detection of cytokines and chemokines. chemokines and cytokines in the kidney and cells were measured using rat TNF-α, IL-6 and McP-1 ELISA kits (RTA00, R6000B and dY3144-05, respectively; R&d Systems, Inc.) according to the procedure recommended by the manufacturer. .. The samples were read at 450 nm within 30 min by Spectramax Microplate Reader (Molecular devices, LLc).

    Article Title: Ligustrazine ameliorates acute kidney injury through downregulation of NOD2-mediated inflammation
    Article Snippet: The second antibodies included HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) (cat. no. SA00001-1, 1:3,000, ProteinTech Group, Inc.) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) (cat. no. SA00001-2, 1:3,000, ProteinTech Group, Inc.). .. Chemokines and cytokines in the kidney and cells were measured using rat TNF-α, IL-6 and MCP-1 ELISA kits (RTA00, R6000B and DY3144-05, respectively; R&D Systems, Inc.) according to the procedure recommended by the manufacturer. .. The samples were read at 450 nm within 30 min by Spectramax Microplate Reader (Molecular Devices, LLC).

    Article Title: Galectin-3 Modulates Microglia Inflammation in vitro but Not Neonatal Brain Injury in vivo under Inflammatory Conditions.
    Article Snippet: The cytotoxicity detection kit (LDH) was bought from Roche Applied Science (Indianapolis, IN, USA) and BCA protein assay kit from Thermo Scientific Pierce (Waltham, MA, USA). .. Mouse IGF-1 and MCP-1 ELISA kits were obtained from R&D Systems (Minneapolis, MN, USA), while Bio-Plex ProTM Mouse cytokine Assay kit (M60-009 RDPD) and Bio-PlexTM200 system were provided by Bio-Rad Laboratories Inc. (Hercules, CA, USA). .. In addition, a SpectraMax® Plus 384 Spectrophotometer from MD (Ramsey, MN, USA) was used.

    Article Title: Role of Human Mesangial-Tubular Crosstalk in Secretory IgA-Induced IgA Nephropathy.
    Article Snippet: .. Enzyme-Linked Immunosorbent Assay To detect TNF-α, transforming growth factor-β1 (TGF-β1) and monocyte chemotactic protein-1 (MCP-1) levels in cell culture supernatants, standard sandwich ELISAs were performed using human TNF-α, TGF-β1, and MCP-1 ELISA kits (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s specifications. .. Real-Time Quantitative PCR Total cellular RNA was prepared using TRIZOL® Reagent (Invitrogen, Carlsbad, CA, USA) and used for cDNA synthesis with a reverse transcription kit (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions.

    Article Title: Butyrate acts as a positive allosteric modulator of the 5-HT transporter to decrease availability of 5-HT in the ileum.
    Article Snippet: State Key Laboratory of Pharmaceutical Biotechnology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China Department of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China Centre for PanorOmic Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China Micon Analytics, Toronto, Canada

    Cytokine Assay:

    Article Title: Galectin-3 Modulates Microglia Inflammation in vitro but Not Neonatal Brain Injury in vivo under Inflammatory Conditions.
    Article Snippet: The cytotoxicity detection kit (LDH) was bought from Roche Applied Science (Indianapolis, IN, USA) and BCA protein assay kit from Thermo Scientific Pierce (Waltham, MA, USA). .. Mouse IGF-1 and MCP-1 ELISA kits were obtained from R&D Systems (Minneapolis, MN, USA), while Bio-Plex ProTM Mouse cytokine Assay kit (M60-009 RDPD) and Bio-PlexTM200 system were provided by Bio-Rad Laboratories Inc. (Hercules, CA, USA). .. In addition, a SpectraMax® Plus 384 Spectrophotometer from MD (Ramsey, MN, USA) was used.



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    BAI increased the cell viability and inhibiting the apoptosis, inflammation, and oxidative stress in ZAS-induced podocyte injury. (A-B) The viability and apoptosis of MPC-5 cells were assessed using CCK-8 (A) and flow cytometry (B) after induction with 10% ZAS for 1 h followed by treatment with 2 µmol/L or 10 µmol/L BAI for 24 h. (C-E) The levels of pro-inflammatory cytokines TNF-α (C), IL-6 (D), <t>and</t> <t>MCP-1</t> (E) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. (F) The levels of ROS in MPC-5 cells were evaluated by flow cytometry following treatment with ZAS and BAI. (G-H) The level of T-SOD (G) and MDA (H) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. Data are represented as mean ± SD ( n = 5 biological repetitions). ns indicated not statistically different, * p < 0.05, ** p < 0. 01, *** p < 0.001.
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    Image Search Results


    BAI increased the cell viability and inhibiting the apoptosis, inflammation, and oxidative stress in ZAS-induced podocyte injury. (A-B) The viability and apoptosis of MPC-5 cells were assessed using CCK-8 (A) and flow cytometry (B) after induction with 10% ZAS for 1 h followed by treatment with 2 µmol/L or 10 µmol/L BAI for 24 h. (C-E) The levels of pro-inflammatory cytokines TNF-α (C), IL-6 (D), and MCP-1 (E) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. (F) The levels of ROS in MPC-5 cells were evaluated by flow cytometry following treatment with ZAS and BAI. (G-H) The level of T-SOD (G) and MDA (H) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. Data are represented as mean ± SD ( n = 5 biological repetitions). ns indicated not statistically different, * p < 0.05, ** p < 0. 01, *** p < 0.001.

    Journal: Renal Failure

    Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

    doi: 10.1080/0886022X.2026.2653954

    Figure Lengend Snippet: BAI increased the cell viability and inhibiting the apoptosis, inflammation, and oxidative stress in ZAS-induced podocyte injury. (A-B) The viability and apoptosis of MPC-5 cells were assessed using CCK-8 (A) and flow cytometry (B) after induction with 10% ZAS for 1 h followed by treatment with 2 µmol/L or 10 µmol/L BAI for 24 h. (C-E) The levels of pro-inflammatory cytokines TNF-α (C), IL-6 (D), and MCP-1 (E) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. (F) The levels of ROS in MPC-5 cells were evaluated by flow cytometry following treatment with ZAS and BAI. (G-H) The level of T-SOD (G) and MDA (H) in MPC-5 cells were evaluated by ELISA following treatment with ZAS and BAI. Data are represented as mean ± SD ( n = 5 biological repetitions). ns indicated not statistically different, * p < 0.05, ** p < 0. 01, *** p < 0.001.

    Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

    Techniques: CCK-8 Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay

    BAI mitigated the inflammation of IMN mice. (A) After being treated with 20 mg/kg and 100 mg/kg BAI, the histopathology in kidney tissue of IMN mice was determined using HE staining. (B) After being treated with BAI, the level of CD68-positive cells in the kidney tissue of IMN mice was determined using IHC. (C-E) After being treated with BAI, the serum levels of TNF-α (C), IL-6 (D), and MCP-1 (E) in IMN mice were detected using ELISA. Data are represented as mean ± SD ( n = 8 biological repetitions). * p < 0.05, ** p < 0. 01, *** p < 0.001.

    Journal: Renal Failure

    Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

    doi: 10.1080/0886022X.2026.2653954

    Figure Lengend Snippet: BAI mitigated the inflammation of IMN mice. (A) After being treated with 20 mg/kg and 100 mg/kg BAI, the histopathology in kidney tissue of IMN mice was determined using HE staining. (B) After being treated with BAI, the level of CD68-positive cells in the kidney tissue of IMN mice was determined using IHC. (C-E) After being treated with BAI, the serum levels of TNF-α (C), IL-6 (D), and MCP-1 (E) in IMN mice were detected using ELISA. Data are represented as mean ± SD ( n = 8 biological repetitions). * p < 0.05, ** p < 0. 01, *** p < 0.001.

    Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

    Techniques: Histopathology, Staining, Enzyme-linked Immunosorbent Assay

    BAI suppressed the inflammation and oxidative stress of ZAS-induced podocytes by inactivating the AGE/RAGE pathway. (A-B) After being 10% ZAS induced for 1 h and 2 µmol/L BAI treated for 24 h, the expression of AGE (A) and RAGE (B) in MPC-5 cells were quantified. (C) After being treated with 2 µmol/L BAI and 2 µg/mL AGE-BSA for 24 h, the expression of RAGE in ZAS-induced MPC-5 cells were quantified using western blot assay. (D) The viability of ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment was determined using CCK-8 assay. (E-G) The levels of pro-inflammatory cytokines TNF-α (E), IL-6 (F), and MCP-1 (G) in ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment were evaluated by ELISA. (H-I) The level of T-SOD (H) and MDA (I) in ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment were evaluated by ELISA. Data are represented as mean ± SD ( n = 5 biological repetitions). ns indicated not statistically different, * p < 0.05, ** p < 0. 01, *** p < 0.001.

    Journal: Renal Failure

    Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

    doi: 10.1080/0886022X.2026.2653954

    Figure Lengend Snippet: BAI suppressed the inflammation and oxidative stress of ZAS-induced podocytes by inactivating the AGE/RAGE pathway. (A-B) After being 10% ZAS induced for 1 h and 2 µmol/L BAI treated for 24 h, the expression of AGE (A) and RAGE (B) in MPC-5 cells were quantified. (C) After being treated with 2 µmol/L BAI and 2 µg/mL AGE-BSA for 24 h, the expression of RAGE in ZAS-induced MPC-5 cells were quantified using western blot assay. (D) The viability of ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment was determined using CCK-8 assay. (E-G) The levels of pro-inflammatory cytokines TNF-α (E), IL-6 (F), and MCP-1 (G) in ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment were evaluated by ELISA. (H-I) The level of T-SOD (H) and MDA (I) in ZAS-induced MPC-5 cells after BAI and AGE-BSA treatment were evaluated by ELISA. Data are represented as mean ± SD ( n = 5 biological repetitions). ns indicated not statistically different, * p < 0.05, ** p < 0. 01, *** p < 0.001.

    Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

    Techniques: Expressing, Western Blot, CCK-8 Assay, Enzyme-linked Immunosorbent Assay

    BAI mitigated the inflammation and oxidative stress of IMN mice via AGE/RAGE pathway. (A) After being treated with 20 mg/kg BAI and 20 mg/kg AGE-BSA, the histopathology in kidney tissue of IMN mice was determined using HE staining. (B) The level of CD68-positive cells in the kidney tissue of IMN mice was determined using IHC. (C-E) The serum levels of TNF-α (C), IL-6 (D), and MCP-1 (E) in IMN mice were detected using ELISA. (F) The LDH level in the kidney tissue of IMN mice was detected using a commercial reagent kit. (G-H) The levels of T-SOD (G) and MDA (H) in kidney tissue of IMN mice was detected by ELISA. Data are represented as mean ± SD ( n = 8 biological repetitions). ** p < 0.01, *** p < 0.001.

    Journal: Renal Failure

    Article Title: Baicalin ameliorates podocyte injury and renal function impairment in idiopathic membranous nephropathy by inhibiting the AGE/RAGE signaling

    doi: 10.1080/0886022X.2026.2653954

    Figure Lengend Snippet: BAI mitigated the inflammation and oxidative stress of IMN mice via AGE/RAGE pathway. (A) After being treated with 20 mg/kg BAI and 20 mg/kg AGE-BSA, the histopathology in kidney tissue of IMN mice was determined using HE staining. (B) The level of CD68-positive cells in the kidney tissue of IMN mice was determined using IHC. (C-E) The serum levels of TNF-α (C), IL-6 (D), and MCP-1 (E) in IMN mice were detected using ELISA. (F) The LDH level in the kidney tissue of IMN mice was detected using a commercial reagent kit. (G-H) The levels of T-SOD (G) and MDA (H) in kidney tissue of IMN mice was detected by ELISA. Data are represented as mean ± SD ( n = 8 biological repetitions). ** p < 0.01, *** p < 0.001.

    Article Snippet: The ELISA and commercial reagent kits used were as follows: TNF-α ELISA kit (E-EL-M3063, Elabscience), MCP-1 ELISA kit (E-EL-M3001, Elabscience), IL-6 ELISA kit (E-EL-M0044, Elabscience), T-SOD ELISA kit (50104ES60, YEASEN, China), MDA ELISA kit for cells ( GMS50099.1 , AIDISHENG, Jiangsu, China), MDA ELISA kit (GMS50099.2, AIDISHENG), C3 ELISA kit (ADS-0550M1, AIDISHENG), AGE ELISA kit (ab238539, Abcam), and lactate dehydrogenase (LDH) activity assay kit (E-BC-K046-M, Elabscience).

    Techniques: Histopathology, Staining, Enzyme-linked Immunosorbent Assay